Utilizing high-throughput sequencing to identify plant pathogens

Description

This project aims to evaluate and develop a high-throughput sequencing-based diagnostic procedure to

• provide an accurate assessment of its pathogen recognition capability in comparison with conventional diagnostic approaches;
• develop processes adapted for both diagnosis and detection that are rapid, accurate, and cost-effective;
• develop an intuitive Web interface to provide for rapid and easy data interpretation, and
• allow for the knowledge transfer and validation of the high-throughput sequencing diagnostic strategy and the integration of a specialized and tested database that will increase data processing speed via a Web interface.

This innovative diagnostic approach will significantly contribute to the development and adoption of diagnostic methods that rely on high-throughput sequencing, thereby simplifying the diagnosis process.

Objective(s)

• Demonstrate that this technique allows for the simultaneous, rapid, and accurate identification of pathogenic organisms responsible for major diseases in field crops, potatoes, and market garden crops.
• Produce an accurate assessment of its pathogen recognition capability.
• Establish procedures adapted to both diagnosis and detection that are rapid, accurate, and cost-effective.
• Develop an intuitive Web interface to provide for rapid and easy data interpretation.