The aim of this project is to use high-throughput sequencing (HTS) techniques to develop a process for detecting and identifying viruses (PDIV). The process will combine HTS with innovative bioinformatic analysis tools so that, using a single analysis, plant pathologists will be able to receive, via a user-friendly interface, a quantitative verdict regarding the presence and identity of all viruses infecting the sample.
To ensure the accurate detection and identification of all raspberry and strawberry viruses, the project will develop
A database with all the HTS sequences will be linked to a database of the agronomic, environmental, and diagnostic parameters for each sample. During trials, these sequence collections and databases will be used to demonstrate the superior diagnostic efficacy of the PDIV compared to reference protocols used by Laboratoire d’expertise et de diagnostic en phytoprotection (LEDP).
An economic analysis will compare the costs of implementing and utilizing the LEDP and PDIV tests, and the benefits derived from using the PDIV.
From 2019 to 2023
Soil health, Pest, weed, and disease control
With high-throughput sequencing, users can identify all viruses infecting a sample with a single analysis.
Ministère de l'Agriculture, des Pêcheries et de l'Alimentation du Québec | Centre de recherche du CHU de Québec-Université Laval | Ferme Onésime Pouliot | Phytoclone
The project consisted of sterilizing spotted wing drosophilas in the laboratory and mass releasing it in crops as a biocontrol method.
Researcher: Annabelle Firlej
Developing a Codling moth control management tool based on an improved formulation of Virosoft CP4.
Exclusion nets have proven to be effective against nearly all of these insect pests, which means that it’s possible to develop apple growing practices in Québec that are not only neonicotinoid free, but also devoid of all pesticides (including acaricides, given that mite problems are a consequence of broad-spectrum insecticide use). Although the net exclusion microsystem studied in Québec since 2012 has demonstrated its effectiveness in controlling insect pests, some issues remain to be studied before it can be unreservedly recommended. Among these are the handling times for the nets, i.e., installation/removal and opening/closing, and the system’s profitability and durability over the long haul for various cultivars.
Researcher: Gérald Chouinard