Colorado potato beetle pesticide resistance: validation of genetic tests for identification

Célia Bordier

450 653-7368
ext 631

Contact Célia Bordier


The Colorado potato beetle (Leptinotarsa ​​decemlineata), by causing plant defoliation, can cause an 80% yield loss in affected fields and thus significantly impact the sector. The most widespread means of control currently is the use of synthetic insecticides. Knowing that the Colorado potato beetle has already shown resistance to 56 active ingredients, it is among the 10 most resistant insects in the world and it becomes difficult to control its populations. This project therefore proposes to establish bioassay protocols to assess the resistance of several Quebec populations, to then determine the molecular origin of these resistances and ultimately transfer a reliable detection tool to the Expertise Laboratory and of Phytoprotection Diagnostics (LEDP).


The main objective of this project is to combine an applied approach using bioassays and a fundamental approach using molecular biology to determine resistance markers and thus equip the LEDP to diagnose resistance of wild populations.

The specific objectives are as follows:

  1. Establish the discriminating dose resulting in 90% mortality in a susceptible Colorado potato beetle population for 12 pesticides.
  2. Validate the use of bioassays in the determination of resistance.
  3. Assess the resistance of wild Quebec populations.
  4. Determine the genes involved in the resistance mechanism of wild populations, using a molecular biology technique.
  5. Transfer the methodology to the Phytoprotection Expertise and Diagnosis Laboratory of MAPAQ.

From 2021 to 2025

Project duration

Market gardening

Activity areas

Pest, weed, and disease control



This project is funded by the ministère de l'Agriculture, des Pêcheries et de l'Alimentation under the Prime-Vert program.

This may interest you

2020-2023 • Market gardening

Evaluation of the agronomic performance of 10 broccoli varieties under commercial production

This project aims to limit the impacts of climate change on broccoli crops by evaluating and documenting the tolerance to climatic stress of 10 broccoli varieties intended for the fresh market.

Researcher: Carl Boivin

Read more about the project

Carl Boivin
2019-2022 • Market gardening

Improving potato crop water-use efficiency through cropping system modifications

Modifying the cropping system design is an effective way to improve potato crop water-use efficiency and, thereby, lessen the risk of crops experiencing water stress.

Researchers: Carl Boivin Luc Belzile

Read more about the project

Carl Boivin
2019-2023 • Market gardening

Using cesium-137 to quantify organic horticultural soil erosion

Project to quantify the long-term (60+ years) severity of erosion of organic horticultural soils.

Read more about the project

Claude Bernard
Marc-Olivier Gasser